Effect of jaw forward distance on forced inspiratory airflow in patients with obstructive sleep apnea hypopnea syndrome / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To study the effect of different jaw forward distance on forced inspiratory airflow(FIF) in non-apnea subjects and patients with obstructive sleep apnea hypopnea syndrome (OSAHS) and to evaluate the effective jaw forward distance for the treatment of OSAHS with the oral appliance.</p><p><b>METHODS</b>FIF was measured in 18 non-apnea subjects and 18 OSAHS patients at supine and lateral body positions with different jaw forward distances (the percentages of maximum jaw forward distance): 0%, 25%, 50% and 75%. FIF were converted to percentage values (FIF%, x(-) ± s) followed by averaged. Then the results were analyzed by one-way analysis of variance and paired t-test with α = 0.05.</p><p><b>RESULTS</b>For non-apnea subjects, there was no significant difference in the FIF values between different jaw forward distances as well as different body positions. For OSAHS patients, the mean FIF% at supine and lateral body positions were 107.1% ± 29.0% and 112.0% ± 33.1% at jaw forward 50%, and were 106.4% ± 20.7% and 116.8% ± 36.4% at jaw forward 75%, respectively, which were significantly higher than those (84.0% ± 18.3% and 98.3% ± 24.0%) at jaw forward 0% or those (92.7% ± 21.8% and 103.7% ± 22.6%) at jaw forward 25%, respectively. But there was no statistical difference in FIF between the two groups of jaw forward 50% and jaw forward 75% and no statistical difference in FIF between supine and lateral body positions in the same forward position.</p><p><b>CONCLUSION</b>Jaw forward 50% is a effective jaw forward distance by oral appliance for the treatment of OSAHS and can improve the airway ventilation in OSAHS patients.</p>

Effect of human semaphorin-3F gene transient transfection on the proliferation of tongue carcinoma cells / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the effect of semaphorin-3F (SEMA-3F) gene transient transfection on the proliferation of Tca8113 tongue carcinoma cells.</p><p><b>METHODS</b>After construction of a full-length SEMA3F expression vector, Tca8113 cells were transient transfected with pEGFP-N1-SEMA3F by Lipofectamine 2000. The expression of SEMA-3F gene was detected by RT-PCR The differences of the expression in the transfected cell groups, empty vector groups and un-transfected cell groups were compared. The survival rates were assayed by methyl thiazolyl tetrazolium (MTT) enzymatic labeling technique. Cell cycle were assayed by flow cytometer (FCM).</p><p><b>RESULTS</b>The gene was transfected into Tca8113 cells. High expression of SEMA-3F was successfully detected in the transfected cell groups after gene transfection. The cell cycle percentage of G1 stage decreased and S stage increased.</p><p><b>CONCLUSIONS</b>SEMA-3F gene transient transfection may inhibit the proliferation of Tca8113 cells.</p>

Application of lateral arm free flap in oral and maxillofacial reconstruction following tumor surgery

To describe the application of lateral arm free flap [LAFF] in reconstruction of defects in the oral and maxillofacial regions following ablative oncological surgery. The study included 16 patients [13 male, 3 female, mean age 56, range 35-69 years]. Sixteen LAFF were harvested to reconstruct defects caused by the dissection of malignant tumors of the oral and maxillofacial regions. The tumor was squamous cell carcinoma of the tongue [6 cases], floor of the mouth [4], retromolar area [3], inner cheek [2], and lower gingival [1]. Flap sizes ranging from 5 _ 7 to 6 _ 9 cm were harvested using a sterile tourniquet for bloodless technique. The anastomoses were carried out using a magnifier or microscope. All donor defects were closed primarily. Fourteen flaps healed without venous insufficiency. One flap, in a female patient, survived with mild local microcirculatory obstruction but that of another female patient developed necrosis. There was no significant complication at the donor sites. The advantages of this flap include anatomically reliable vascular supply, accessible donor site, and the aesthetic quality of donor tissue is good. Compared with the radial artery, the posterior radial collateral artery is a nonessential vessel of the arm. The disadvantages are the relatively smaller vessel size for anastomosis and thicker subcutaneous tissue. For the repair of moderate-sized defects of the maxillofacial area, especially in male patients, the LAFF can be recommended